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anti cd5 vioblue clone rea421  (Miltenyi Biotec)


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    Miltenyi Biotec anti cd5 vioblue clone rea421

    Anti Cd5 Vioblue Clone Rea421, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rea421/CD5+Antibody%2C+anti-mouse%2C+REAfinity/pmc11231613-43-0-4
    Average 92 stars, based on 20 article reviews
    anti cd5 vioblue clone rea421 - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Increased peritoneal B1-like cells during acute phase of human septic peritonitis"

    Article Title: Increased peritoneal B1-like cells during acute phase of human septic peritonitis

    Journal: iScience

    doi: 10.1016/j.isci.2024.110133


    Figure Legend Snippet:

    Techniques Used: Recombinant, Blocking Assay, Software

    Related Articles

    Staining:

    Article Title: Single-cell gene and isoform expression analysis reveals signatures of ageing in haematopoietic stem and progenitor cells.
    Article Snippet: Red blood cell depletion was performed with ammonium chloride lysis (STEMCELL Technologies), and lineage-negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies). .. The lineage-depleted cells were stained with the following fluorophoreconjugated monoclonal antibodies: Cd105-PE, clone MJ7/18, Miltenyi; Cd4Vioblue, clone REA604, Miltenyi; Cd11b-Vioblue, clone REA592, Miltenyi; Cd117Pe Vio770, clone REA791, Miltenyi; Cd8a-Vioblue, clone 53-6.7, Miltenyi; Cd50Vioblue, clone REA421, Miltenyi; Cd45R-Vioblue, clone REA755, Miltenyi; GR1Vioblue, clone REA810, Miltenyi; Sca-APC, clone REA422, Miltenyi; Cd48-APC Cy7, clone HM48-1, Miltenyi; Cd150-BV510, clone TC15-12F12, Cd34-PeCy5, MEC147, Miltenyi. .. Approximately 10,000 LK (Lin−, Cd117+) cells per sample were sorted using the BD FACSMelody cell sorter (BD Biosciences, San Jose, California) into 1× PBS containing 4% BSA.

    Article Title: Single-cell gene and isoform expression analysis reveals signatures of ageing in haematopoietic stem and progenitor cells
    Article Snippet: Red blood cell depletion was performed with ammonium chloride lysis (STEMCELL Technologies), and lineage-negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies). .. The lineage-depleted cells were stained with the following fluorophore-conjugated monoclonal antibodies: Cd105-PE, clone MJ7/18, Miltenyi; Cd4-Vioblue, clone REA604, Miltenyi; Cd11b-Vioblue, clone REA592, Miltenyi; Cd117-Pe Vio770, clone REA791, Miltenyi; Cd8a-Vioblue, clone 53-6.7, Miltenyi; Cd50-Vioblue, clone REA421, Miltenyi; Cd45R-Vioblue, clone REA755, Miltenyi; GR1-Vioblue, clone REA810, Miltenyi; Sca-APC, clone REA422, Miltenyi; Cd48-APC Cy7, clone HM48-1, Miltenyi; Cd150-BV510, clone TC15-12F12, Cd34-PeCy5, MEC147, Miltenyi. .. Approximately 10,000 LK (Lin−, Cd117+) cells per sample were sorted using the BD FACSMelody cell sorter (BD Biosciences, San Jose, California) into 1× PBS containing 4% BSA.

    Article Title: Comprehensive single-cell gene and isoform expression analysis reveals signatures of ageing in haematopoietic stem and progenitor cells
    Article Snippet: Red blood cell depletion was performed with ammonium chloride lysis (STEMCELL Technologies) and lineage negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies). .. The lineage depleted cells were stained with following fluorophore-conjugated monoclonal antibodies: Cd105-PE, clone MJ7/18, Miltenyi; Cd4-Vioblue, clone REA604, Miltenyi; Cd11bVioblue, clone REA592, Miltenyi; Cd117-Pe Vio770, clone REA791, Miltenyi; Cd8a-Vioblue, clone 53-6.7, Miltenyi; CD50-Vioblue, clone REA421, Miltenyi; CD45R-Vioblue, clone REA755, Miltenyi; GR1-Vioblue, clone REA810, Miltenyi; Sca-APC, clone REA422, Miltenyi; CD48-APC Cy7, clone HM48-1, Miltenyi; CD150-BV510, clone TC15-12F12, CD34-PeCy5, MEC147, Miltenyi. .. Approximately 10,000 LK (Lin-, CD117+) cells per sample were sorted using the BD FACSMelody cell sorter (BD Biosciences, San Jose, California) into 1 x PBS containing 4% BSA.

    Article Title: Combined single-cell gene and isoform expression analysis in haematopoietic stem and progenitor cells
    Article Snippet: Red blood cell depletion was performed with ammonium chloride lysis (STEMCELL Technologies) and lineage negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies). .. The lineage depleted cells were stained with following fluorophore-conjugated monoclonal antibodies: Cd105-PE, clone MJ7/18, Miltenyi; Cd4-Vioblue, clone REA604, Miltenyi; Cd11b-Vioblue, clone REA592, Miltenyi; Cd117-Pe Vio770, clone REA791, Miltenyi; Cd8a-Vioblue, clone 53-6.7, Miltenyi; CD50-Vioblue, clone REA421, Miltenyi; CD45R-Vioblue, clone REA755, Miltenyi; GR1-Vioblue, clone REA810, Miltenyi; Sca-APC, clone REA422, Miltenyi; CD48-APC Cy7, clone HM48-1, Miltenyi; CD150-BV510, clone TC15-12F12, CD34-PeCy5, MEC147, Miltenyi. .. Approximately 10,000 LK (Lin-, CD117+) cells per sample were sorted using the BD FACSMelody cell sorter (BD Biosciences, San Jose, California) into 1 x PBS containing 5% BSA.

    Bioprocessing:

    Article Title: Single-cell gene and isoform expression analysis reveals signatures of ageing in haematopoietic stem and progenitor cells.
    Article Snippet: Red blood cell depletion was performed with ammonium chloride lysis (STEMCELL Technologies), and lineage-negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies). .. The lineage-depleted cells were stained with the following fluorophoreconjugated monoclonal antibodies: Cd105-PE, clone MJ7/18, Miltenyi; Cd4Vioblue, clone REA604, Miltenyi; Cd11b-Vioblue, clone REA592, Miltenyi; Cd117Pe Vio770, clone REA791, Miltenyi; Cd8a-Vioblue, clone 53-6.7, Miltenyi; Cd50Vioblue, clone REA421, Miltenyi; Cd45R-Vioblue, clone REA755, Miltenyi; GR1Vioblue, clone REA810, Miltenyi; Sca-APC, clone REA422, Miltenyi; Cd48-APC Cy7, clone HM48-1, Miltenyi; Cd150-BV510, clone TC15-12F12, Cd34-PeCy5, MEC147, Miltenyi. .. Approximately 10,000 LK (Lin−, Cd117+) cells per sample were sorted using the BD FACSMelody cell sorter (BD Biosciences, San Jose, California) into 1× PBS containing 4% BSA.

    Article Title: Single-cell gene and isoform expression analysis reveals signatures of ageing in haematopoietic stem and progenitor cells
    Article Snippet: Red blood cell depletion was performed with ammonium chloride lysis (STEMCELL Technologies), and lineage-negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies). .. The lineage-depleted cells were stained with the following fluorophore-conjugated monoclonal antibodies: Cd105-PE, clone MJ7/18, Miltenyi; Cd4-Vioblue, clone REA604, Miltenyi; Cd11b-Vioblue, clone REA592, Miltenyi; Cd117-Pe Vio770, clone REA791, Miltenyi; Cd8a-Vioblue, clone 53-6.7, Miltenyi; Cd50-Vioblue, clone REA421, Miltenyi; Cd45R-Vioblue, clone REA755, Miltenyi; GR1-Vioblue, clone REA810, Miltenyi; Sca-APC, clone REA422, Miltenyi; Cd48-APC Cy7, clone HM48-1, Miltenyi; Cd150-BV510, clone TC15-12F12, Cd34-PeCy5, MEC147, Miltenyi. .. Approximately 10,000 LK (Lin−, Cd117+) cells per sample were sorted using the BD FACSMelody cell sorter (BD Biosciences, San Jose, California) into 1× PBS containing 4% BSA.

    Article Title: Comprehensive single-cell gene and isoform expression analysis reveals signatures of ageing in haematopoietic stem and progenitor cells
    Article Snippet: Red blood cell depletion was performed with ammonium chloride lysis (STEMCELL Technologies) and lineage negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies). .. The lineage depleted cells were stained with following fluorophore-conjugated monoclonal antibodies: Cd105-PE, clone MJ7/18, Miltenyi; Cd4-Vioblue, clone REA604, Miltenyi; Cd11bVioblue, clone REA592, Miltenyi; Cd117-Pe Vio770, clone REA791, Miltenyi; Cd8a-Vioblue, clone 53-6.7, Miltenyi; CD50-Vioblue, clone REA421, Miltenyi; CD45R-Vioblue, clone REA755, Miltenyi; GR1-Vioblue, clone REA810, Miltenyi; Sca-APC, clone REA422, Miltenyi; CD48-APC Cy7, clone HM48-1, Miltenyi; CD150-BV510, clone TC15-12F12, CD34-PeCy5, MEC147, Miltenyi. .. Approximately 10,000 LK (Lin-, CD117+) cells per sample were sorted using the BD FACSMelody cell sorter (BD Biosciences, San Jose, California) into 1 x PBS containing 4% BSA.

    Article Title: Combined single-cell gene and isoform expression analysis in haematopoietic stem and progenitor cells
    Article Snippet: Red blood cell depletion was performed with ammonium chloride lysis (STEMCELL Technologies) and lineage negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies). .. The lineage depleted cells were stained with following fluorophore-conjugated monoclonal antibodies: Cd105-PE, clone MJ7/18, Miltenyi; Cd4-Vioblue, clone REA604, Miltenyi; Cd11b-Vioblue, clone REA592, Miltenyi; Cd117-Pe Vio770, clone REA791, Miltenyi; Cd8a-Vioblue, clone 53-6.7, Miltenyi; CD50-Vioblue, clone REA421, Miltenyi; CD45R-Vioblue, clone REA755, Miltenyi; GR1-Vioblue, clone REA810, Miltenyi; Sca-APC, clone REA422, Miltenyi; CD48-APC Cy7, clone HM48-1, Miltenyi; CD150-BV510, clone TC15-12F12, CD34-PeCy5, MEC147, Miltenyi. .. Approximately 10,000 LK (Lin-, CD117+) cells per sample were sorted using the BD FACSMelody cell sorter (BD Biosciences, San Jose, California) into 1 x PBS containing 5% BSA.

    Flow Cytometry:

    Article Title: Adaptation by naïve CD4 + T cells to self-antigen–dependent TCR signaling induces functional heterogeneity and tolerance
    Article Snippet: Cell sorting was performed using Beckman Coulter MoFlo XDP, BD FACS Aria II or Sony SH800 sorters. .. The following antibodies were used for flow cytometry analysis: CD4 clone RM4-5 (Biolegend and BD); CD5 clone 53-7.3 (BioLegend); CD5 clone REA421 (Miltenyi); CD25 clone PC61 (BioLegend); CD44 clone IM7 (BioLegend); CD45.1 clone A20 (BioLegend); CD45.2 clone 104 (BioLegend and eBioscience); CD62L clone MEL-14 (BioLegend and eBioscience); CD69 clone H1.2F3 (BioLegend); TCR-β clone H57-597 (BioLegend); TCR Vα2 clone B20.1 (BioLegend and BD); TCR Vα11.1 clone RR8-1 (BioLegend); Ly6C clone AL-21 (BD); PD-1 clone 29F.1A12 (BioLegend). .. LIVE/DEAD fixable near-IR dead cell stain (catalog # {"type":"entrez-nucleotide","attrs":{"text":"L10119","term_id":"497765","term_text":"L10119"}} L10119 , Thermo Fisher Scientific) was used for discrimination of viable cells.



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    Image Search Results


    Journal: iScience

    Article Title: Increased peritoneal B1-like cells during acute phase of human septic peritonitis

    doi: 10.1016/j.isci.2024.110133

    Figure Lengend Snippet:

    Article Snippet: anti-CD5-VioBlue clone REA421 , Miltenyi Biotec , cat#130-123-287; RRID: AB_2811489.

    Techniques: Recombinant, Blocking Assay, Software

    Immune cell profiling of kidney tissues: (A) representative images of massive infiltrates in the kidneys from old RAGE −/− male (upper panel) and female (lower panel) mice, and the infiltrates are stained positive for B-cell marker b220 left (green) and right. H&E of the same animal. (B) Immune cells were isolated from the whole kidney and analyzed by FACS from 62–68 weeks old wild type and RAGE −/− ( n = 4) (male and female combined) mice. Representative FACS plots. (C) Quantification of B cells (B220), T cells (CD3), NK cells (NK1.1), and myeloid cells (CD11b). Populations were calculated as % of live CD45+ as parent gating. (D) B cells were further analyzed for B-1a (CD19+, CD43+, and CD5+) and B-1b (CD19+ and CD43+) subtypes. Population was calculated as % of live C19+ as parent gating. B220, CD19, and B-1a B cells are the dominant population in RAGE −/− , and p -values were calculated by the non-parametric t -test (Mann–Whitney test). Data are shown as mean ± SD; p- value: 0.05*, 0.01**, 0.001***, and 0.0001****.

    Journal: Frontiers in Physiology

    Article Title: RAGE is a critical factor of sex-based differences in age-induced kidney damage

    doi: 10.3389/fphys.2023.1154551

    Figure Lengend Snippet: Immune cell profiling of kidney tissues: (A) representative images of massive infiltrates in the kidneys from old RAGE −/− male (upper panel) and female (lower panel) mice, and the infiltrates are stained positive for B-cell marker b220 left (green) and right. H&E of the same animal. (B) Immune cells were isolated from the whole kidney and analyzed by FACS from 62–68 weeks old wild type and RAGE −/− ( n = 4) (male and female combined) mice. Representative FACS plots. (C) Quantification of B cells (B220), T cells (CD3), NK cells (NK1.1), and myeloid cells (CD11b). Populations were calculated as % of live CD45+ as parent gating. (D) B cells were further analyzed for B-1a (CD19+, CD43+, and CD5+) and B-1b (CD19+ and CD43+) subtypes. Population was calculated as % of live C19+ as parent gating. B220, CD19, and B-1a B cells are the dominant population in RAGE −/− , and p -values were calculated by the non-parametric t -test (Mann–Whitney test). Data are shown as mean ± SD; p- value: 0.05*, 0.01**, 0.001***, and 0.0001****.

    Article Snippet: Following antibodies were used in the staining: CD45R/B220 (RA3-6B2), CD19 (eBio1D3), CD43 (S7) from eBioscience/Invitrogen, CD19 (1D3), CD3 (500A2), NK1.1 (PK136), CD11b (M1/70) from BioLegend, CD5 (REA421) and CD45 (REA737) from Miltenyi Biotec B.V. & Co. KG, FSC and SSC signals were acquired to eliminate dead cells and doublets.

    Techniques: Staining, Marker, Isolation, MANN-WHITNEY